Major Instruments (CAI)

Nuclear Magnetic Resonance (NMR)

Nuclear Magnetic Resonance (NMR)

NMR spectroscopy is a research technique that exploits the resonance phenomenon of certain atomic nuclei in compounds. This is necessary and powerful measurement for structural analyses of organic molecules consisting of carbon, hydrogen and nitrogen, and so on. It is also effective to observe dynamic behaviors of the molecules.

In CAI, four machines of high-resolution NMR (400~600 MHz) are used actively. ECS400 and ECA500 are used for standard solution-state measurement. Advanced solid-state measurements can be performed on ECX400 and ECZ600R.

Models
Instrument Managers
  • Hyuma Masu
  • Katsuhiko Moriyama
  • Keisuke Iida
  • Daiki Mitsumoto
  • Takahiro Ohkubo
  • Keisuke Ueda
  • Hirofumi Dohi
  • Tatsuya Seki

Time-domain NMR

After the nuclear magnetic resonance, the nuclear spins experience the relaxation depending on the molecular motions. MV-25 has the low-field parmanent magnets and detects the relaxation times of either solid- or liquid-state samples, which makes us assess the kinetic properties of materials easily.

Models
Instrument Managers
  • Daiki Mitsumoto

Mass Spectrometer (MS)

Mass Spectrometer (MS)

Mass Spectrometry is an analytical technique that measures the mass-to-charge ratio of charged (ionized) particles. It is an effective analytical method to elucidate the elemental composition and structure of compounds from very small amount of the sample.

AccuTOF is time-of-flight mass spectrometer and is equipped with electron ionization (EI), field ionization (FI), and field desorption (FD) ion sources. Exactive is electronic FT (Orbitrap) mass spectrometer and is equipped with electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI), atmospheric pressure photoionization (APPI), and direct analysis in real time (DART) ion sources. LTQ Orbitrap XL is hybrid FT-MS (linear ion trap and Orbitrap) and is equipped with nano-ESI ion source. Exact mass measurements are available by all instruments.

Models
Instrument Managers
  • Sayaka Kado

X-ray Diffractometer (XRD)

Single crystal X-ray Diffractometer

Single crystal X-ray structural analysis is a method using XRD from single crystal to determine the 3D positions of atoms and molecules in the crystal. It is the strictest method for determination of the 3D structure of compounds including large molecules as proteins. Our diffractometers are equipped with cryostat system.

D8 VENTURE is equipped with the Micro-Focus Xray tubes (Mo/Cu switchable) and the highly sensitive CPAD-type large-area detector, which makes it possible to measure smaller crystals than 0.1 mm.

The Cambridge Structural Database (CSD) is available for XRD users.

Models
Instrument Managers
  • Hyuma Masu

Powder X-ray Diffractometer

Powder X-ray diffraction is the method using XRD from powder samples to identify the including compounds and to observe structural mutation. It is useful for investigations of properties of organic or inorganic materials.

D8 ADVANCE is suitable for multi-purpose of powder X-ray diffraction. The 1-dimensional detector is rapid and increases measured intensities. A chamber for variable-temperature (+450~-193 °C) is available.

Models
Instrument Managers
  • Hyuma Masu
  • Takashi Kojima
  • Masatoshi Sakai

Elemental Analyzer (EA)

Elemental Analyzer (EA)

EA is a fundamental analytical method to determine the types and weight percent of each element in a compound. The most common type of EA is the combustion analysis for carbon (C), hydrogen (H), and nitrogen (N) by combusting the sample under conditions where the resulting combustion products can be quantitatively analyzed. This type is especially useful for determination of structure or purity of organic compounds.

In CAI, CHN analysis is performed by PE2400ll and CE-440F. Electronic micro balances and a glove box are set up to weight and treat samples accurately.

Models
Instrument Managers
  • Hyuma Masu
  • Sayaka Kado
  • Shoji Matsumoto

Electronic Microscope (EM)

Scanning Electron Microscope (SEM)

SEM is a type of electron microscope that images a sample by scanning it with an electron beam. The producing signals (secondary and backscattered electrons) contain information about the surface and structure. SEM has been widely used for observations of various samples at micrometer ~ nanometer scale.

JSM-6510A is equipped with an energy dispersive X-ray spectrometer (EDS) that provides elemental analysis. It also equips optional cryo-stage for biological/organic samples and backscattered electron detector.

Models
Instrument Managers
  • Sayaka Kado
  • Kenjiro Higashi
  • Hirofumi Hidai

Transmission Electron Microscope (TEM)

TEM is a microscopy technique whereby an electron beam is transmitted through an ultra-thin sample and interacts with the sample to provide interference images. The surface and internal structures of materials can be observed by TEM at micrometer ~ angstrom scale.

H-7650 can select acceleration voltage (60~120 kV) and two lens mode (high-contrast/ high-resolution). It is useful for both biological/organic samples and inorganic materials at nanometer scale.

Models
Instrument Managers
  • Hyuma Masu

Field Emission Transmission Electron Microscope (FE-TEM)

FE-TEM has a field emission electron gun to obtain fine high-resolution (magnification) TEM images.

JEM-2100F is used for a scanning transmission electron microscopy (STEM), electron diffraction (ED), and energy dispersive X-ray spectroscopy (EDS). It also equips an optional cryo-transfer holder.

Models
Instrument Managers
  • Hyuma Masu
  • Tomonori Ohba
  • Tsuyoshi Morita
  • Kenjiro Higashi

Instruments for Electron Microscopy/Surface Processing

Models
Instrument Managers
  • Hyuma Masu (Etching)
  • Sayaka Kado (Au/Os)
  • Naofumi Uekawa (Os)
  • Takaomi Itoi (PIPS)

Spectrometer

Microspectroscope

Microscopic spectrometer (microspectroscope) provides spectral information of samples on a defined small area for chemical and physicochemical analyses.

MSV-370 can measure absorption or reflection spectra in a wide range of ultraviolet-visible-near-infrared wave lengths (250~2000 nm) with an aperture. The aperture size is variable and can be fixed from 10 μm × 10 μm.

Models
Instrument Managers
  • Hyuma Masu
  • Tomonori Ohba

Infrared Microspectroscope

Energy of infrared light is equivalent to rotational or vibrational movements of interatomic bonds in molecules. The infrared absorption spectroscopy is used to identification and structural analysis of chemical compounds. Moreover, microscopic spectrometer provides spectral information for defined small areas of inhomogeneous samples.

FT/IR-4200ST is a fourier transform infrared spectrometer. It can measure the transmission absorption spectra of the wide range of wave number (7800~350 cm-1) in a short time. IRT-5000 is a microscope connected to the infrared spectrometer. It can measure absorption or reflection spectra with an aperture and performs ATR measurement.

Models
Instrument Managers
  • Hyuma Masu

Photo-Luminescence Quantum Yields Measurement System

The quantum yield of emission (photo luminescence) is the ratio of photons absorbed by fluorophore to photons emitted through fluorescence or phosphorescence. Typically, the quantum yields have been recorded by the comparative method, which involve the use of standard samples.

The Quantaurus-QY enables absolute measurements of the quantum yields of liquid, powder, and films by using integral sphere without standard samples. Excitation wavelength dependence of the quantum yields and color coordinate (X, Y) can be also measured in simple way.

Models
Instrument Managers
  • Kazuki Nakamura
  • Hyuma Masu

Other Instruments

Zeta-Potential and Particle Size Analyzer

Size distribution of particles and zeta potential of them or solid surfaces become more and more important for researches and applications, because they provide information about average size, colloidal stability, or surface property.

ELSZ-1000ZSCK can measure the particle size distribution of colloidal nanoparticles by dynamic light scattering and the zeta potential of them or flat solid surfaces by electrophoretic light scattering.

Models
Instrument Managers
  • Hyuma Masu
  • Daiki Mitsumoto
  • Michinari Kohri

Static and Dynamic Light Scattering Spectrophotometer

Among various methods for measuring molecular weights of polymers, static light scattering is the best to determine the absolute values without any standard materials. In addition, radii of gyration and second virial coefficients are derived, and they indicate some information about structures and characteristics of polymer solutions.

DLS-8300CU, which have the goniometer moiety, can measure the absolute molecular weights from the dependence of scattered light strength on the angles and the concentrations (static light scattering) and the particle sizes from the fluctuations in scattered light (dynamic light scattering).

Models
Instrument Managers
  • Daiki Mitsumoto

Electron Spin Resonance

ESR is a research technique that exploits the resonance phenomenon of certain unpaired electron spin in materials (e.g. transition metal ion and organic free radical). This is necessary and powerful measurement for reaction process analysis, defect assessment, labeled compound detection, and so on.

Although conventional JES-TE200 has old operation system without personal computer, our instrument had been upgraded to use it. This expansion provided easier operation, analysis, and simulation.

Models
Instrument Managers
  • Hyuma Masu